vitro transcription Search Results


vitro transcription kit  (TaKaRa)
95
TaKaRa vitro transcription kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Vitro Transcription Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
vitro transcription kit - by Bioz Stars, 2026-03
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in vitro transcription kit  (Boehringer Mannheim)
90
Boehringer Mannheim in vitro transcription kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Transcription Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
in vitro transcription kit - by Bioz Stars, 2026-03
90/100 stars
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in vitro transcription kit  (Promega)
90
Promega in vitro transcription kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Transcription Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
in vitro transcription kit - by Bioz Stars, 2026-03
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in vitro coupled transcription-translation reticulocyte lysate system  (Promega)
90
Promega in vitro coupled transcription-translation reticulocyte lysate system
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Coupled Transcription Translation Reticulocyte Lysate System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
in vitro coupled transcription-translation reticulocyte lysate system - by Bioz Stars, 2026-03
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in vitro transcription/translation coupled system  (Promega)
90
Promega in vitro transcription/translation coupled system
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Transcription/Translation Coupled System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
in vitro transcription/translation coupled system - by Bioz Stars, 2026-03
90/100 stars
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hela extract stop solution  (Promega)
90
Promega hela extract stop solution
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Hela Extract Stop Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
hela extract stop solution - by Bioz Stars, 2026-03
90/100 stars
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vitro transcription kit  (Enzo Biochem)
90
Enzo Biochem vitro transcription kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Vitro Transcription Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vitro transcription kit/product/Enzo Biochem
Average 90 stars, based on 1 article reviews
vitro transcription kit - by Bioz Stars, 2026-03
90/100 stars
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in vitro transcription  (Promega)
90
Promega in vitro transcription
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Transcription, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in vitro transcription/product/Promega
Average 90 stars, based on 1 article reviews
in vitro transcription - by Bioz Stars, 2026-03
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in vitro translation assays  (Promega)
90
Promega in vitro translation assays
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Translation Assays, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in vitro translation assays/product/Promega
Average 90 stars, based on 1 article reviews
in vitro translation assays - by Bioz Stars, 2026-03
90/100 stars
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genechip in vitro transcript labeling kit  (Enzo Biochem)
90
Enzo Biochem genechip in vitro transcript labeling kit
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
Genechip In Vitro Transcript Labeling Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genechip in vitro transcript labeling kit - by Bioz Stars, 2026-03
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t7 or sp6 transcriptase  (Promega)
90
Promega t7 or sp6 transcriptase
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
T7 Or Sp6 Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
t7 or sp6 transcriptase - by Bioz Stars, 2026-03
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in vitro transcription/translation system  (Promega)
90
Promega in vitro transcription/translation system
In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro <t>transcription.</t> ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)
In Vitro Transcription/Translation System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in vitro transcription/translation system/product/Promega
Average 90 stars, based on 1 article reviews
in vitro transcription/translation system - by Bioz Stars, 2026-03
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Image Search Results


In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro transcription. ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Multiplex CRISPR/Cas9 gene-editing platform in oil palm targeting mutations in EgFAD2 and EgPAT genes

doi: 10.1186/s43141-022-00459-5

Figure Lengend Snippet: In vitro assay to evaluate cleavage efficiency by the Guide-it sgRNA screening kit. ( a ) PCR products from the amplification of EgFAD2 (lane 1: sgRNA1, lane 2: sgRNA2) and EgPAT (lane 3: sgRNA3, lane 4: sgRNA4) sgRNA templates at 130 bp for in vitro transcription. ( b ) PCR products are amplified from oil palm genomic DNA containing sgRNA target sites; the PCR templates of candidate sgRNAs are then in vitro combined with a recombinant Cas9 for a fragment cleavage site ( c ). EgFAD2-T1 (lane 1), EgFAD2-T2 (lane 2), EgPAT-T1 (lane 3), and EgPAT-T2 (lane 4) represent the PCR fragments with 921 bp, 986 bp, 903 bp, and 978 bp length, respectively. Cleavage fragments were present for all sgRNAs; they were cleaved into two fragments by the sgRNA/Cas9 complex: 503 bp and 395 bp for EgFAD2-T1; 559 bp and 404 bp for EgFAD2-T2; 531 bp and 349 bp for EgPAT-T1; and 287 bp and 668 bp for EgPAT-T2. Lane C is a control fragment that has a size of 614 bp and cleaved DNA of 350 bp and 264 bp. Lane W is a negative control, and lane M is a 1 kb plus DNA marker (Invitrogen)

Article Snippet: The sgRNAs designed for targeting the EgFAD2 and EgPAT genes were evaluated based on target cleavage efficiency using the Guide-it™ sgRNA In Vitro Transcription kit and the Guide-it™ Screening Systems Kit (Takara Bio Inc.) according to the manufacturer’s instructions.

Techniques: In Vitro, Amplification, Recombinant, Control, Negative Control, Marker